Palm Oil Decanter Cake Wastes as Alternative Nutrient Sources and Biomass Support Particles for Production of Fungal Whole-Cell Lipase and Application as Low-Cost Biocatalyst for Biodiesel Production

This is the first report on possible use of decanter cake waste (DCW) from palm oil industry as alternative nutrient sources and biomass support particles for whole-cell lipase production under solid-state fermentation (SSF) by newly isolated fungal Aspergillus sp. MS15 their application a low-cost environment-friendly biocatalyst biodiesel production. The results found that DCW supplemented with 0.1% K2HPO4, 0.05% MgSO4·7H2O, 1% peptone 2% urea pH adjusted to 6.0 was optimal moisture content temperature 60% 37.5 °C, respectively. Environmentally friendly production, through either esterification or transesterification using immobilized biocatalyst, optimized. reaction both reactions 37 °C. effectively esterified oleic acid into >95% yield water activity at 0.71 an methanol molar ratio 2:1, also transesterified 0.81 3:1. fuel properties produced are close international standards. These have shown circular utilization mill effective may contribute greatly sustainability renewable bioenergy